[{
  "sequence": {
    "name": "pDEST15",
    "sequence": "ATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTCCATGGTCGAATCAAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATCTAGAGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTTGATTCGACCCGGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATATCCACAGGACGGGTGTGGTCGCCATGATCGCGTAGTCGATAGTGGCTCCAAGTAGCGAAGCGAGCAGGACTGGGCGGCGGCCAAAGCGGTCGGACAGTGCTCCGAGAACGGGTGCGCATAGAAATTGCATCAACGCATATAGCGCTAGCAGCACGCCATAGTGACTGGCGATGCTGTCGGAATGGACGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGCCAGGACCCAACGCTGCCCGAGATGCGCCGCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTCTGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAGTGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACGCCAGCAAGACGTAGCCCAGCGCGTCGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGATCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGG",
    "circular": true,
    "sequenceType": "Nucleotide"
  },
  "annotations": [
    {
      "name": "RBS",
      "type": "RBS",
      "intervals": [
        {
          "min": 90,
          "max": 96,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"123000003"
        }
      ]
    },
    {
      "name": "T7 primer",
      "type": "primer_bind",
      "intervals": [
        {
          "min": 25,
          "max": 44,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"2100000"
        },
        {
          "name": "Sequence",
          "value": "TAATACGACTCACTATAGGG"
        },
        {
          "name": "Hairpin Tm",
          "value": "None"
        },
        {
          "name": "Tm",
          "value": "50.3"
        },
        {
          "name": "Self Dimer Tm",
          "value": "None"
        },
        {
          "name": "%GC",
          "value": "40.0"
        }
      ]
    },
    {
      "name": "T7 reverse primer",
      "type": "primer_bind_reverse",
      "intervals": [
        {
          "min": 2557,
          "max": 2576,
          "direction": "Left",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"2110000"
        },
        {
          "name": "Sequence",
          "value": "TAGTTATTGCTCAGCGGTGG"
        },
        {
          "name": "Hairpin Tm",
          "value": "None"
        },
        {
          "name": "Tm",
          "value": "57.7"
        },
        {
          "name": "Self Dimer Tm",
          "value": "6.8"
        },
        {
          "name": "%GC",
          "value": "50.0"
        }
      ]
    },
    {
      "name": "pBR322 origin",
      "type": "rep_origin",
      "intervals": [
        {
          "min": 4238,
          "max": 4911,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"2540000"
        }
      ]
    },
    {
      "name": "Invitrogen vector 576",
      "type": "source",
      "intervals": [
        {
          "min": 1,
          "max": 7013,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"576"
        }
      ]
    },
    {
      "name": "T7 transcription terminator",
      "type": "terminator",
      "intervals": [
        {
          "min": 2518,
          "max": 2646,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"2630000"
        }
      ]
    },
    {
      "name": "bla promoter",
      "type": "promoter prokaryotic",
      "intervals": [
        {
          "min": 3134,
          "max": 3232,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"2330000"
        }
      ]
    },
    {
      "name": "T7 promoter",
      "type": "promoter prokaryotic",
      "intervals": [
        {
          "min": 25,
          "max": 41,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"2370000"
        }
      ]
    },
    {
      "name": "Amp(R)",
      "type": "CDS",
      "intervals": [
        {
          "min": 3233,
          "max": 4093,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"1020000"
        }
      ]
    },
    {
      "name": "GST tag (no stop)",
      "type": "CDS",
      "intervals": [
        {
          "min": 105,
          "max": 776,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"1165000"
        }
      ]
    },
    {
      "name": "Cm(R)",
      "type": "CDS",
      "intervals": [
        {
          "min": 1025,
          "max": 1684,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"1080000"
        }
      ]
    },
    {
      "name": "ccdB",
      "type": "CDS",
      "intervals": [
        {
          "min": 2026,
          "max": 2331,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"1060000"
        }
      ]
    },
    {
      "name": "ROP",
      "type": "CDS",
      "intervals": [
        {
          "min": 5282,
          "max": 5473,
          "direction": "Left",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"1270000"
        }
      ]
    },
    {
      "name": "initiation ATG",
      "type": "CDS",
      "intervals": [
        {
          "min": 105,
          "max": 107,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": "invitrogen",
          "value": "\"123000000"
        }
      ]
    },
    {
      "name": "attR2",
      "type": "attR2",
      "intervals": [
        {
          "min": 2371,
          "max": 2495,
          "direction": "Left",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": ".hidden_geneious",
          "value": "true"
        }
      ]
    },
    {
      "name": "attR1",
      "type": "attR1",
      "intervals": [
        {
          "min": 793,
          "max": 917,
          "direction": "Right",
          "truncatedMin": false,
          "truncatedMax": false
        }
      ],
      "qualifiers": [
        {
          "name": ".hidden_geneious",
          "value": "true"
        }
      ]
    }
  ],
  "metadata": {}
}]